HighPrep PCR (50 mL)
The HighPrep™ PCR is a post PCR clean up system based on paramagnetic bead based technology designed for an efficient purification and tight size selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles; and highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications. This protocol can be used for manual procedure as well as guideline for adapting the kit to automatic liquid handling instruments.
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